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[[http://www.fungifun.org/contact.htm | Pfpics:brief.gif]]
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(:title Le clonage pour les nuls:)

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!Le clonage pour les nuls
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'-dead link reports, comments and suggestions welcome any time-'
[[http://www.fungifun.org/contact.htm | Pfpics:brief.gif]]

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Pfpics:shroom_zwerg.gif
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Les instructions suivantes vont vous montrer comment cloner un champignon sur du agar.

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Prenez un jolie et vigoureux champignon.

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[[Cloning:DSCF0032.JPG|Cloning:DSCF0032_small.JPG]]

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Sectionnez le champignon en deux dans le sens de la longueur.

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[[Cloning:DSCF0033.JPG|Cloning:DSCF0033small.JPG]]

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Brulez le scalpel jusqu'à ce que la lame devienne rouge.
Laissez reposer quelques secondes.

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[[Cloning:DSCF0035.JPG|Cloning:DSCF0035small.JPG]]

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Prelevez un bout à la base du chapeau, mesurant environ 2 x 2 x 2 mm.

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[[Cloning:DSCF0037.JPG|Cloning:DSCF0037small.JPG]]

[[Cloning:DSCF0038.JPG|Cloning:DSCF0038small.JPG]]

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Transferrez sur du agar.

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[[Cloning:DSCF0039.JPG|Cloning:DSCF0039small.JPG]]

[[Cloning:DSCF0040.JPG|Cloning:DSCF0040small.JPG]]

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Page last modified on January 17, 2007, at 10:55 PM