Crumble and pulverize the dried mycelial material and combine each 100 mg of this material with 10 ml of methanol. Place the flask in a hot water bath for four hours. Filter the liquids with suction through a filter paper in a buchner funnel with Celite to prevent clogging. Collect and save the filtrate liquids. Heat the slurry (the mush in the filter paper) two more times in methanol as before, filter, and accumulate the liquids of the three extractions. To be certain that all of the alkaloids have been extracted do a small extraction with a portion of the used slurry and test with Keller's reagent (glacial acetic acid, ferrous chloride, and concentrated sulfuric acid). If there is a violet indication, alkaloids are still present and further extraction is in order.
In an open beaker evaporate the liquids to total dryness with a hot water bath or by applying a hair dryer. Be certain that all traces of methanol have been removed. The remaining residue should contain 25-50 percent psilocybin/psilocin mixture. Greater purification can be achieved, but would require other solvents and chromatography equipment and is hardly necessary.
Each 100 grams of dried mycelium should yield about 2 grams of extracted material. This should contain at least 500 mg of psilocybin/psilocin mixed or about fifty 10 mg doses. Theoretically psilocin should have the same effect upon the user as psilocybin. The only difference between the two is that the later has a phosphate bond which disappears immediately after assimilation in the body. In other words, in the body psilocybin turns into psilocin. Psilocybin is a fairly stable compound, but psilocin is very susceptible to oxidization. It is best to keep the extracted material in a dry air tight container under refrigeration. A sack of silica-gel can be placed in the container to capture any moisture that may enter.
The standard dose of psilocybin or psilocin for a 150 lb person is a 6-20 mg dose. We will figure the average dose as 10 mg. The crude alkaloid extraction process given here yields a brownish crystalline powder that is at least 25 percent pure. Each mason jar should contain at least 50 grams of wet mycelium. After drying this would be about 5 grams of material. The crude material extracted from this should contain 25-30 mg of psilocybin/ psilocin or roughly 2-3 hits. This yield may very to some extent depending upon several factors. Many of these species contain less of these alkaloids than dose Psilocybe cubensis and the alkaloidal content of this species may very in different strains. Cultivation conditions have alot to do with yield too. Higher temperatures (75 degrees F.) cause more rapid growth but lesser psilocybin content than do lower temperatures (70 degrees F.) One must test each new batch of extracted material to determine the proper distribution of dosages. Depending on the potency of the mycelia and how well the extraction was conducted the dose may range between 25 and 100 mg. Also bear in mind that the dose varies for different individuals.
Paul Stamets - 1996 - book - "PSILOCYBIN MUSHROOMS of the WORLD".
Quote - page 50-51 "Another method I have seen is to soak crushed mushrooms in 75+% ethanol. AFter two to three days, the roughage can be filtered, leaving a dark- blue elixir that can be decanted accordingly. For every fresh 5 grams of mushrooms, 25-30 milliliters of alcohol is recommended. Psilocybin and psilocin dissolve into this solven, and the alcohol also acts as a preservative. I really don't have much experience with this technique, but have talked to people who say it is their preferred method. SOme call this "blue juice."
This technique describes how to extract psilocybin from magic mushrooms with pure 190 proof ethyl alcohol and make a magic mushroom liqueur of concentrated psilocybin to effect a powerful psychedelic dose as potent as desired. The entire process involves only the shrooms and alcohol. The alcohol is untainted with chemicals and poisons because it can be easily acquired from a liquor store (United States) either over the counter (in some states) or with a special permit (most states - see end of article section - "procuring 190 proof ethyl alcohol from a liquor store").
1. Acquire quality psilocybe cubensis shrooms (harvested before or just as the veils open and cool dried with desiccant). The more shrooms used in the beginning, the more potent the concentration can be when finished. Use at least several grams of dried shroom material to make the process worthwhile and effective. The shrooms need to be thoroughly dry (rock hard) to allow pulverization. To pulverize the shrooms, put them into a small strong zip lock plastic bag (freezer bag), cover the bag with a magazine (for protection of the bag) and pound it with the rubber heel of a large shoe. Or, powder them in a small canister type coffee bean grinder.
In a heat resistant soaking vessel (pyrex glass), combine the shroom powder with several times its volume with 190 proof Everclear (ethanol). This is the "slurry". Place the soaking vessel in a pan of boiling water. Raising the soaking vessel off the bottom of the hot water pan is a good idea for preventing serious sticking of the good extracts. The slurry will start to boil. Turn the water boiling pan heat down and let the slurry sit for a few hours at a warm-hot temp. Alcohol boils at a lower temp than water. Watch the temperatures closely. Things can get totally out of hand and ruined very quickly without close attention paid.
While the slurry is still hot, filter it through filter paper. This is probably the most important part. A good filtration will be efficient and will keep most of the shroom material out, making for a clean extraction (clean of shrooms that is - but heavy on psilocybin). A small lab type vacuum pump powered bottle top filtering funnel with filter disk holder makes it all really easy and fast, with little waste. That is why this extraction idea is really only for the fanatics.
Collect and save the filtrate liquids. Heat the slurry (the mush in the filter paper) one or two more times with the 190 proof as before, filter, and accumulate the liquids of the extractions. The photos at the top are of extractions done twice.
Inexpensive dust-pollen masks make excellent filters for the slurry. These are available at hardware, drug and paint stores. They are usually white or tan colored, fit over the nose and mouth and are held on to the face by a rubber band attached to the filter. Fashion the filter over the mouth of a drinking glass. Squeeze the filter and slurry to extract the alcohol. There are many details to deal with, but doing it once reveals them all. Experience is the best teacher. Store the extracted alcohol in a fresh bottle.
Combine the alcohol extracts into a glass. Place a small electric fan (small desk clip on fans are perfect) near the glass and point the air flow directly down into the glass until the surface of the alcohol ripples. This will speed the evaporation and concentration. The process will take several hours. The more alcohol extract - the longer the evaporation time. As the alcohol evaporates and the level recedes down into the glass, wash the residue that adheres to the inside of the glass back into the solution. Any fumes that are generated will be harmless because the alcohol is a non poisonous drinkable spirit. Keep flames away from the solution - pure alcohol is very flammable.
One can also use heat to evaporate and concentrate the elixir. Use a double boiler type of set up to heat and evaporate off the alcohol to concentrate the elixir.
The concentrated shroom liqueur will have a pungent mushroomy aroma (like fungi perfume). Also, a white crystalline kind of precipitate will form in the alcohol elixir (see above photo). Store it in small screw cap bottles or vials in the freezer. Alcohol doesn't freeze solid and will remain liquid.
First, call a well stocked liquor store and ask if they have 190 proof ethyl alcohol. Full service liquor stores supply hospitals and laboratories with 190 - 200 proof ethyl alcohol.
If a permit is needed, call the state liquor board (usually in the State Capital) and ask for an application to get an ethyl alcohol permit. The fee is 5 or 10 dollars. On the application will be a question asking what the use of the alcohol will be. Write what they more or less want to hear. State that the use of the alcohol will be for "non-toxic surface sterilizing plus herb extraction - preservation - tincture - and perfume making" (or something to that effect). The poison warnings on the alcohol bottles and this idiotic red tape are just bureaucratic nonsense that results in the state making a big fat bundle off of the sale.
1. Use warm-hot temps when soaking the initial slurry (shroom-alki).
Use the hot water bath idea from the Gottlieb tek below. Avoid
hot bottomed slurry soak vessels. The good stuff can bake on
and stick very easily.
2. A good filter is a must. Lab quality filter paper helps for a cleaner extract (less shroom stuff). The fanatic should get a little bottle top vacuum filtering funnel with a hand squeeze vacuum pump and fine slow flow filtering papers. (science supply - not cheap - but affordable for the fanatics - look for the 47 millimeter filter sized set ups - small but perfect for this).
3. When filtering the slurry, do it while it is hot.
4. The crystals when heated in the initial slurry are free base molecules. In the final liqueur on cool down, the free base molecules will coalesce and form crystals. It takes a day or two for the process to be complete. The smaller the final amount of liqueur, the easier it is for the molecules to meet each other and combine. When you get your final magic liqueur, the free base psilocybin will coaslesce and form whitish crystals. At first they might look like whitish glue, but they transform in solution to hard crystals.
As an example, one can start with 20 grams of dried shrooms. After the filtration of the hot slurry, the resultant liqueur should be put into an evaporation vessel and with a fan blowing air across the mouth of the vessel, the liqueur should be evaportated down to about 50 milliliters. Then, in a double boiler, heat the small amount of liqueur to put the crystals and extract back into a cloudy solution. Then while it is hot, dispense 10 cc of the liqueur into waiting small storage jars with watertight caps. Each small jar is allowed to cool, the cap is put on and the jar is placed into the freezer for storage. Then when it is time to trip, the desired jars are removed from the freezer, allowed to warm to room temps, the lids taken off, a small fan set up blowing air across the jars mouths and the liquere evaporated off to a manageble "hit". The small jars then become adminstration "spoons" - where the entire contents (alcohol - water - and extract) can be polished off with the tongue.
PF has monitored the WEB for 7 years and has seen no posts concerning this easy way to extract and seriously concentrate the shrooms magic. PF has always said that this extraction tek solves the "problems" of impotent or mediocre magic shrooms.
Then, finally, these posts have appeared at the MYCOTOPIA interactive myco hobbiest web site:
By Searcher (Novice) on Saturday,
November 16, 2002 - 06:29 pm:
My most exciting piece of beginner's luck came when I got rid of some cakes that seemed stalled in their development (and/or had really uneven surface colonization) by splitting up and drying the mycelium for use in place of mushroom dust to create the liquer described in the following link:
By letting the resulting liquer continue to dry out, I wound up with amber colored crystals that could be crushed and placed in gel caps. A few hundred milligrams of this extract sent a curious fruend of mine into orbit, and now he's been bugging the shit out of me to butcher ALL of my cakes to concentrate on this extract and forget about trying to grow shrooms. He claims the trips he got were much "cleaner" (whatever that means) and that the stuff certainly tastes better than the shrooms themselves.
As I recall, there were about 6 or 7 jarsthat seemed to have good mycelial development, but colonization was very uneven, or so slow, that I abandoned them to put better jars in my grow chamber. Rather than just tossing out the slow jars, I decided to break them up and try Prof PF's extraction technique. By the way, the alcohol seemed to absorb a lot of water from the air - it no longer smelled of alcohol, and the drying process slowed down a lot just before the amber tar and then the crystals appeared. I used no heat, but kept a large fan blowing on the liquid over a large, shallow cookie sheet.
Behold, cystals - little white snowflakes - just using 99% isopropyl---
Here are the results from my first methanol soak:
Yielded 3.85 grams of crystals from 8 ounces of mycelium. But wait, there's more! Remember we're doing three, consecutive soaks. Looks like we'll be getting even more residues out of soaks 2 & 3:
Miscellaneous notes on the methanol: You'll note that this residue us lighter in color than residue gleaned from the water soaks. Also, this residue comes out dryer - no intermediate "tar" stage.
Almost any liquid will extract something from the shrooms themselves or the mycelium. Even water will work, producing a brown syrup that can be dried into a sticky tar. Only the acetone was a bust in extracting the magic - but it does extract some other crud that might make it a good pre-wash for more serious extractions. Recapping the results of the tests using other spirits, 99% isopropyl, 151 proof ethyl alcohol, and 99.9% methanol - methanol was the hands down winner for getting the crystals. Once the pretty white crystals have dried, they can be re-dissolved in grain alcohol for a potent elixer. The crystals tend to absorb water from the air unless they are kept in a heavily dessicated chamber.
I tested my 20g dried shroom, 24cc Solution. AWESOME is all i got to say I had one of the BEST trips ever. We started with 1cc each and started feeling the effects almost immediately. Within 10min we where having the full level 1 experience. We decided to take another CC, Took us to level 2.... Within 10 min. By this time it was hard to keep track of time. But we did 1 more CC and that was all we needed.
NO unpleasent side effects... No upset stomach, or dizziness... nothing negative. It was VERY euphoric, very clear. We were tripping hard, yet we could think very clearly. Couldn't always speak clearly but the thoughts were profound and clear. The visuals where awesome.... everything was so alive and beautiful. The moon was full... so that made for a magical evening.
The greatest thing about the extract was the control we had over the dosage. Each dosage was equal with magic content. Unlike when you eat the shrooms themselves where the magical content will be different from shroom to shroom. So, dosage isn't quite as accurate. I've split a bag of shrooms with a friend before and got off way more than he did... on the same shrooms. With the extract we both knew we were getting the same amount.
I've had tea, chocolate shrooms, cooked with magic.. even psycedellic coffee, and this is by far the best method of consumption. I will be testing how long the extract lasts in potentcy.
I had this in the freezer for a week... and it was still very potent.
Anyway guys... there is my experience with the liquid. Freakin awesome!
Thank you PROF and Searcher for bringing that tech. out to mycotopia. I've never heard of liquid shrooms until now! WOW!
I'll post my other experiments with this in time.
Peace & Light to all!!