Mushroom cultivation > Cultivation

sclerotia sterilization

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enviromentaladditive:
Is it possible to sterilize jars of rye seed in a pressure cooker without tyvec or polyfill lids, could I use a hole in the lid with foil wrapped over it?

IndiaShroomer:
Yes, you can sterilize that way. You only want to be careful that the foil doesnt get loose when the pressure builds up, if the steam gets into the jar, the moisture content will be too high.

enviromentaladditive:

--- Quote ---Yes, you can sterilize that way. You only want to be careful that the foil doesnt get loose when the pressure builds up, if the steam gets into the jar, the moisture content will be too high.
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--- End quote ---

Does the lid need to be shut tight, should the hole be punched in the jar before or after pressure sterilization?

IndiaShroomer:

--- Quote ---
--- Quote ---Yes, you can sterilize that way. You only want to be careful that the foil doesnt get loose when the pressure builds up, if the steam gets into the jar, the moisture content will be too high.
[snapback]499[/snapback]
--- End quote ---

Does the lid need to be shut tight, should the hole be punched in the jar before or after pressure sterilization?
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--- End quote ---

lid needs to be shut tight, punch holes before you sterilize. If you try this after sterilization, you may end up introducing contaminants in your otherwise sterile substrate.

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