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Author Topic: agar  (Read 1584 times)

jrfungi

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agar
« on: March 09, 2009, 07:16:02 PM »

  i am cloning a PE strain this week to agar. After i do the clone do i put the petri dish into the incubator or just in a room temp. dark place? Do i need to wrap saran wrap around the dish?

  I want to do the "super myc" tek i saw on a video and then another set of dishes just regular.

1. After i transfer the the best rizo MYC to another dish is dish "1" ruined, or can i continue to grow it and transfer later?

2. Will a small piece if agar work alone in grain or is a transfer to LC a must?

3. Do you wait until a "regular" dish is completely white before any sort of transfer?

Thanks again in advance!!!

JR
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psilocybin warrior

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Re: agar
« Reply #1 on: March 10, 2009, 12:30:28 PM »

agars are very sensitive to contam's, keep it at room temp, dark place, saran wrap around the dish if u wanna play it safe.

1- Once you get the myc you want, the rest of the agar is worthless.  Remember to cut the mycelium you wan't into 3's for 3 sep dishes incase of contams.

2- Yes, transfer to LC is better tho.  What you could do is if you end up with 2 or 3 uncontam'd dishes. Transfer one to grains, transfer the other to LC, and do what you wan't with the other one.

3- You can if you wan't too, its not totally necessary.

PW
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