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Author Topic: Agar cleaning process  (Read 2727 times)

tenaglia

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Agar cleaning process
« on: April 04, 2009, 02:57:51 AM »

How do you obtain a clean culture? I tried to select apparently clean mycelium to inoculate a new petri dish, but I always carry some pennicillium or something like that; should I bath the wedge in a peroxide solution? I thought It shouldn't be neccessary   :huh:
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psilocybin warrior

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Re: Agar cleaning process
« Reply #1 on: April 04, 2009, 08:21:57 AM »

Did you boil your potatos first? if not, boil them instead of the peroxide dip.


PW
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tenaglia

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Re: Agar cleaning process
« Reply #2 on: April 04, 2009, 08:26:09 AM »

I use ready made potato dextrose agar, I sterilize it for 20 minutes then I leave the fresh dishes in the incubator for 4-5 days to see if they are poured clean.
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psilocybin warrior

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Re: Agar cleaning process
« Reply #3 on: April 04, 2009, 08:33:36 AM »

Wait, I dont think I understand.... your not innocing them right after sterilization but waiting 4-5 days?
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tenaglia

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Re: Agar cleaning process
« Reply #4 on: April 05, 2009, 04:58:47 AM »

Right, I dissolve 39g potato-dextrose with 5g agar in one liter water, I adjust the ph, I sterilize it for 20 min, I pour it in 20 dishes, I wait a couple of days to see if I worked sterile enough, then I innoculate them :ph34r: If you do not wait, you have chance to innoculate a petri which isn't sterile anymore...

The biologist I work with told me to do so, he works with tissue-culture (orchids, meat-eaters, aquarium plants and much more)
« Last Edit: April 05, 2009, 05:10:55 AM by tenaglia »
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psilocybin warrior

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Re: Agar cleaning process
« Reply #5 on: April 05, 2009, 01:28:45 PM »

I've never bothered waiting whats steril is steril, maybe the waiting a few day's is allowing it to produce another kind of bacteria, its cool ur working with a biologist, however this is mycology not biology.

Pw
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