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Author Topic: Inoculating a WET Jar  (Read 2239 times)

PyramidHead1128

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Inoculating a WET Jar
« on: April 16, 2009, 07:36:56 PM »

I've inoculated 2 of my 4 jars today.
I boiled them for an hour and a half and i left them in the pot until they were room temperature.  I took them out and put them in my sterile room.  As soon as i tore off the tin foil on top of the jars, i noticed that they still were wet and the masking tape i put on the holes were wet.  i looked in the jars and saw spots of water in the jars. 
I only inoculated 2 of them because i wasn't sure i was suppose to inoculate them when their wet.  I'm saving the 2 others for later until they dry or something.
Did I do this right or what.  Are they suppose to be wet or dry?
Thanks.
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Azurascender

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Re: Inoculating a WET Jar
« Reply #1 on: April 16, 2009, 09:12:26 PM »

How high did you fill the pot during sterilization? It sounds to me that you got a little splash up from the boiling water. You only need to fill the water level an inch or so from the bottom of the jars. You should be fine, just wipe them off with a clean towel, then after that wipe the top with rubbing alcohol, before doing that make sure you oust the area so you don't get any contaminates on your project. You did everything right though it sounds like....
« Last Edit: April 16, 2009, 09:22:39 PM by Azurascender »
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Chloroform and a dish towel, yeah that will keep them quiet....