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Author Topic: sterilizing the needle  (Read 6108 times)

spaceboi

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sterilizing the needle
« on: January 17, 2010, 07:56:24 PM »

can you use a candel flam to sterilize the needle to innocculate the jars
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moorhS

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Re: sterilizing the needle
« Reply #1 on: January 17, 2010, 08:22:45 PM »

first time I did it I used a alcohol lamp but my success rate wasn't as good as with a tourch (probably due to the thick syringe tip I was using)... I would say with a candle you would get wax on your syringe tip and possibly get contams but not sure about that... just make sure whatever you use that it gets the needle red hot... I usually cool with alcohol soaked paper towel
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dub504

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Re: sterilizing the needle
« Reply #2 on: January 17, 2010, 10:12:43 PM »

I use a gas stove. I think a candle flame might be a kind of dirty flame source, a lot of carbon, which would probably stick to the needle.
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coyoteyogi

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Re: sterilizing the needle
« Reply #3 on: January 18, 2010, 06:00:45 AM »

It takes a steady hand with a candle. You want to put the needle at the top of the small blue flame. Otherwise you get the black coating.
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fattrout

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Re: sterilizing the needle
« Reply #4 on: January 18, 2010, 02:47:35 PM »

i us just 91% or higher alcohol on a cotton ball. wipe down the needle then wait about 5 sec. and your ready.... :mellow:
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Helo

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Re: sterilizing the needle
« Reply #5 on: January 28, 2010, 10:39:16 PM »

I just used alcohol for my first time sterilizing and innoculating. I haven't had any contams yet.  ^_^
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lun0s

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Re: sterilizing the needle
« Reply #6 on: January 30, 2010, 09:59:15 AM »

When I think of it I flame sterilized after each inoculation, and ALL but one jar were contaminated... I had black residue left on my needle from the flame sterilization. I held the needle on the orange part of a lighter flame, could that be the reason?
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fattrout

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Re: sterilizing the needle
« Reply #7 on: January 31, 2010, 08:28:07 AM »

i'm sure that a lighter or candle can be use. but both of those will leave soot. thats why alcohol is best. it burns very clean.....
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ImAFungi

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Re: sterilizing the needle
« Reply #8 on: January 31, 2010, 10:39:38 AM »

If you go to walmart, they have these really cheap burners. Like its a small can that has some paste. You just light, wait a bit, and it's good to use. Its all blue flame, which you want. So I would just go buy that. It's like $5.

            -ImAFungi
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shroomzer

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Re: sterilizing the needle
« Reply #9 on: January 31, 2010, 04:04:04 PM »

Alcohol.  Cheapest, easiest, cleanest way to sterilize. Period.
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MT

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Re: sterilizing the needle
« Reply #10 on: February 01, 2010, 06:21:16 PM »

Buy or make an alcohol burner.
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-CAP KING-

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Re: sterilizing the needle
« Reply #11 on: February 03, 2010, 08:04:49 AM »

yoyo

Im about to start so im gathering my disinfectants. can i use surgical spirit to sterilise the needle?
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dub504

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Re: sterilizing the needle
« Reply #12 on: February 03, 2010, 10:02:20 AM »

I think shroomzer said it best...
Alcohol.  Cheapest, easiest, cleanest way to sterilize. Period.
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MT

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Re: sterilizing the needle
« Reply #13 on: February 03, 2010, 04:44:35 PM »

I don't know what "surgical spirit " is. I always use 91% alcohol.
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