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Author Topic: strain isolation?  (Read 3858 times)

F.W.Despot

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strain isolation?
« on: May 10, 2010, 05:24:08 PM »

when isolating a strain is done only by creating LC or taking the myc. and replicating it or can i just wait for fruiting and harvest spores from the biggest fastest growing caps?
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dub504

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Re: strain isolation?
« Reply #1 on: May 10, 2010, 07:00:12 PM »

Strain isolation is usually done through agar work, cloning a particular fruit and selecting rizomorphic mycelium which you could grow out in an LC or by inoculating sterile grains. Any spores from a chosen fruit will have different genetic traits at play and that is where the mycologist would continue to refine the strain toward further stability.
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substrate

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Re: strain isolation?
« Reply #2 on: May 18, 2010, 04:40:35 PM »

Dub is right on for real lab-type isolation.  You can blender-tek your freakiest fruit into an LC, but you'll likely get poor spore production from the clones.  The spores will also be working against the isolate, trying to bring the variety back to the strain.

Nature wins out over nurture most of the time.  Otherwise I'd blend my Ecuadore and Garden Giant myc and grow the worlds biggest magic fruit...  Imagine 5 pound Eqs growing in your back yard!
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F.W.Despot

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Re: strain isolation?
« Reply #3 on: May 18, 2010, 06:43:44 PM »

so just out of curiosity dose this tek (http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek) basically render the same results as the blender tek?
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substrate

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Re: strain isolation?
« Reply #4 on: May 23, 2010, 05:31:14 PM »

I've never tried this particular tek myself, but vie heard you can do the same thing just buy adding pure water, shaking the jar, then pulling the excess water out with a syringe.  The water will have bits of myc in it, which you can use for another set of jars. 
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dub504

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Re: strain isolation?
« Reply #5 on: May 24, 2010, 01:00:19 PM »

That is called a "slurry".
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