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Author Topic: liquid culture  (Read 5054 times)

fungiguy

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liquid culture
« on: December 19, 2010, 08:03:59 AM »

I started some lc about 11 days ago. It appears as though it worked. I used honey. How long can my jar survive in the fridge??
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darksyde

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Re: liquid culture
« Reply #1 on: December 19, 2010, 09:34:20 PM »

6 months or longer as posted by some people at shroomery/mycotopia.. Just make sure its sealed good to keep out the bad guys  :angry:
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Azurascender

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Re: liquid culture
« Reply #2 on: December 21, 2010, 10:59:19 AM »

How did you prep your jar lids? Did you do the whole tyvek top scenario or filter patches? Medi tape?
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Chloroform and a dish towel, yeah that will keep them quiet....

Azurascender

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Re: liquid culture
« Reply #3 on: December 22, 2010, 12:40:10 PM »

Actually I was curious of your tek alltogether haha...
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Chloroform and a dish towel, yeah that will keep them quiet....

darksyde

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Re: liquid culture
« Reply #4 on: December 22, 2010, 01:35:51 PM »

Azurascender,

Give this a read.. Works great!

http://www.shroomery.org/forums/showflat.php/Number/5874305
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fungiguy

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Re: liquid culture
« Reply #5 on: December 30, 2010, 06:57:49 AM »

I just read that tek. I did not do it right. There is white clumps floating in my jar, but it is very cloudy. I am gonna follow this new tek. Trial and error!!!
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substrate

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Re: liquid culture
« Reply #6 on: March 20, 2011, 12:21:52 PM »

When LC sits for a while myc begins to clump together, dont worry about that at all.  If your mix is just honey and water, it shouldn't be too cloudy.  If you have a hole in the top of your jar, your brew might be fermenting - making alcohol and killing myc.  That happened to me the first time I tried it (using honey and potato water).

Now I just pour boiling water til the jar overflows, add honey, let cool a bit.  Add spores or myc and put the lid on tight - no hole in it - if it can't breath it can't ferment.
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Dr.Shroom

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Re: liquid culture
« Reply #7 on: April 18, 2011, 07:24:09 PM »

hello im new to the growing side of mycological work but i have made several practice spore syringes,
and can identify about 8 subs around the area i live in. Im interested to know what a
Liquid Culture Jar is and what its purpose is as some kind of test.
could someone explain how and why a liquid culture is done?

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Vandal_xx

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Re: liquid culture
« Reply #8 on: April 21, 2011, 11:26:30 AM »

Liquid Culture (LC) is made in order to have a jar of mycelium floating in water so you can use it to start grow projects. It will aso cononize the substrate faster than a spore print.
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