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Author Topic: Cloning / Isolating vs. Multispore  (Read 5330 times)

Baphom3t

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Cloning / Isolating vs. Multispore
« on: July 26, 2007, 06:39:59 AM »

During this last project I have ran across the issue of performance.

Now with multispore innoc I am getting the idea that pinsets may not become all that good. i.e alot of side pinning and spotty pinning at the center.
But with Cloning / Isolating the performance can be ten fold.

Is the reason for this due to the choosing of the most vigorous Myc growth?
and that you are choosing Myc that has already become dikaryonic?

Is the reason that multispore flushes are the way they are due to the spores starting out as monokaryonic which takes time and energy from the nutes to become dikaryonic?
Which would result in a lesser flush?

Comments are greatly welcome.

Baph

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anno

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Re: Cloning / Isolating vs. Multispore
« Reply #1 on: July 27, 2007, 08:25:10 AM »

>alot of side pinning and spotty pinning at the center.

That's a humidity problem, at the sides of the container towards the container wall the humidity is higher.

The reason why advanced growers prefer isolates is that they deliver predictable and uniform results. Once you isolate your strain, preferably by cloning a mushroom which shows desirable traits, you can give the isolate just the right environmental conditions and substrate  to get the most out of it.

This doesn't mean multispore inoculations can't perform good, too.

What Stamets writes (in GGMM):

"Theoretically, the germination of spores in mass creates multitudes of strains that will compete with one another for nutrients. This has been long accepted as one of the Ten Commandments of Mushroom Culture. Scientists in China, whose knowledge had not been contaminated by such preconceptions first developed spore-mass inoculation techniques to an industrial level.

Only recently have Western mycologist recognized that a large community of spore matings behaves quite differently than paired individuals. San Antonio and Hanners (1984) are some of the first Western mycologists to realize that grain spawn of Oyster mushrooms could be effectively created via spore-mass inoculation.
The most aggressive strains out-race the least aggressive strains to capture the intended habitat.

Recent studies have shown that these aggressive strains overpower and invade the cellular network of competing strains.
Dr. Alan Rayner (1988) in studies at the University of Bath described this form of genetic theft as "non-self fusions" between genetically different mycelial systems within the same species. This ability to adapt has made fungi one of the most successful examples of evolution in the biological arena.
Spore-mass fermentation techniques are not yet widely used by North American or European cultivators. Concern for preserving strain stability, lack of experience, equipment, and intellectual conflicts are contributing factors. In mushroom culture, intransigence to new ideas has prevailed, often because the slightest variation from the norm has resulted in expensive failures. Since the health of any economy is based on its diversity, the emergence of organically minded gourmet mushroom growers is creating a fertile intellectual habitat for many innovative technologies."
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Baphom3t

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Re: Cloning / Isolating vs. Multispore
« Reply #2 on: July 28, 2007, 06:24:51 AM »

Ok , would the humidity be to high or low?
My RH is 92-96%
I'm wondering if the trays I am using could be a factor,They are aluminum and light can get to the sides.
I havent seen any condensation along the sides of the tray.

I'm gonna also guess that it's due to the casing that I am using as well.
My last few grows I've tried to get a better pinset.This one seems to be my best one,but I'm thinking I may need to do some tweaking on my next project.

Baph
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anno

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Re: Cloning / Isolating vs. Multispore
« Reply #3 on: July 28, 2007, 09:31:39 AM »

>Ok , would the humidity be to high or low?

Too low in the pinning phase.
After pining, 90% is good.

>I'm wondering if the trays I am using could be a factor,They are aluminum and light can get to the sides.

Could be, yes.
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Baphom3t

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Re: Cloning / Isolating vs. Multispore
« Reply #4 on: July 28, 2007, 09:42:20 AM »

So I can drop my Rh ?
I'm going into my second flush.

Tupperware here I come  -_-

would it being 100% coir The main factor with pinset?
I have done 50/50 and 60/40.
Better pinset but most aborted due to RH I think.
I plan on trying your topsoil/limeflour mix in my next casing.
Any suggestions?
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