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Author Topic: New Here & Q For Anno  (Read 2127 times)

Wormy

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New Here & Q For Anno
« on: August 02, 2007, 05:47:19 PM »

Hi, lurked this site off and on for a few years now, checking it occasionally after it first went up. Just realized there is now a forum. Nice looking site.

My question to Anno is about the Tyvek test where he uses it compared to polyfill :

Did you (Anno) use 1 layer of Tyvek?

And you say reduce the size of the lid opening to a 1/2 inch hole?

I wasnt sure if you meant 1/2 inch smaller than the cutout lids you used, or 1/2 inch hole.

Any feedback appreciated.
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Wormy

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anno

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Re: New Here & Q For Anno
« Reply #1 on: August 03, 2007, 07:06:27 AM »

Welcome!

I used 1 layer of tyvek.
And yes, I recommended to use an app. 1/2 inch hole to prevent drying out of the substrate.

Since then, I have been using both tyvek and polyfil lids and have found that for me polyfil works better. Tyvek jars would contaminate from time to time with green mold, whereas polyfil filter lid jars hardly every have this problem.

So if you ask me what I recommend today, definitely the polyfil filter.
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Wormy

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Re: New Here & Q For Anno
« Reply #2 on: August 03, 2007, 02:00:31 PM »

Thanks for the reply Anno.

Have you ever inoculated with a syringe through the polyfill? Or do you add a seperate injection hole? Or just take the lid off, inject using flowhood? I know you do grain to grain and agar usually, but curious if you ever tried with syringe.

I appreciate the feedback.
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Wormy

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anno

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Re: New Here & Q For Anno
« Reply #3 on: August 06, 2007, 09:01:52 AM »

>Have you ever inoculated with a syringe through the polyfill?
No.

>Or do you add a seperate injection hole?
Yes.

>Or just take the lid off, inject using flowhood?
Yes, also possible if you have a flowhood.

>I know you do grain to grain and agar usually, but curious if you ever tried with syringe.
I did, sure.
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