inoculation spores question

Started by hauntedgraffiti, May 15, 2008, 01:41:28 PM

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hauntedgraffiti

So I know that you need to put 1-1.5 ml of the spores into each jar. But since there are four holes, how do I balance that out? How many ml. should I put in each hole?

And also, to sterilize the needle, could I use a regular pocket lighter, or would I need something else?

CAPTROB

>>So I know that you need to put 1-1.5 ml of the spores into each jar. But since there are four holes, how do I balance that out?

You don't have to be exact on inoculating each hole, just put a little of solution in each of them. 

>>How many ml. should I put in each hole?

You don't need much, 10cc can do about 8 or 9  1/2 pint jars

>>And also, to sterilize the needle, could I use a regular pocket lighter, or would I need something else?

You can use a lighter...Get the needle red hot then wipe it with some 91% Isopropyl Alcohol...(rubbing alcohol) You can find this in the first aid area of any store.

anno

Quote from: hauntedgraffiti on May 15, 2008, 01:41:28 PM
So I know that you need to put 1-1.5 ml of the spores into each jar. But since there are four holes, how do I balance that out? How many ml. should I put in each hole?

Thanks to the achievements of algebra we can figure this pretty easy nowadays.

1 ml / 4 holes = 1/4 ml/hole = 0.25 ml/hole

psilocybin warrior