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#51
PF - Tek / Fruiting Chamber
Last post by greenleaf19 - October 26, 2013, 02:13:23 PM
Hello all. I just found this amazing community and am falling in love. I recently moved into my basement and can no longer let my FC sit out in an ideal room temp variable. I have used an incubator process to colonize my cakes, and am curious if anyone has any ideas to re amp this design of an incubator, http://www.fungifun.org/English/Pftek , or some cost effective way to control the environment around my cakes.

I figure just leaving the temp of the water consistent and have my FC lifted off of the bottom of the inner tub for bottom air flow, but this completely eliminates almost all air flow in the FC and ill only be able to fan from top not able to get a fan inside.

Any ideas to elaborate the design, or anything new. thanks all
#52
PF - Tek / None of my spore prints worked
Last post by Polybius - October 23, 2013, 02:28:42 PM
I inoculated about 40 jars using syringes I made from prints from my first grow and none of them have colonized. One of them has four quarter sized white patches of mycelium which appeared overnight, but that one has completely ceased it's growth. Not a single one of any of the others shows any signs at all of growth, and it's been three weeks now.

I prepared the substrate the exact same way I did last time. I'm very certain the substrate isn't the issue, because I did a test with a jar that I did not sterilize, and it's beginning to grow some sort of random white mold inside it. So clearly the substrate allows for some level of growth.

I thought that maybe the water levels of the substrate were off, or something, but out of forty jars, you'd think that that would mean that some would simply colonize faster than others, or not at all.

I also experimented with using a small amount of spore solution compared to a lot of spore solution. On two jars, I used an entire syringe. Nothing.

This led me to believe that the problem had to be with the spores themselves. I researched how to make a liquid culture to test further. Sterilize a jar, boil water, fill the jar with the water, mix in about a tablespoon of clear honey, and then add a few cc's of spore solution. Every report I've read online indicates that spores in this environment will produce mycelium with a matter of days. Guess what. Nothing.

So I've narrowed the issue, I THINK, down to the spores themselves. Now, clearly SOME of them are still viable. One single jar had growth before it stopped. The thing is, I used that syringe on many other jars, and they produced nothing.

I made spore prints on both card stock as well as tin foil, and have used both those to make syringes, so I don't think it has to do with what I made the print on. The only thing that I failed to do when making the prints was letting them dry for two days after removing the caps before wrapping them in foil. COuld this be the issue?

I can only assume that the issue is something to do with the spores. When I made the syringes, I made sure to boil the water for at least ten minutes to get rid of impurities. And I let the syringes filled with water cool in the fridge to make sure that hot water would not damage the spores. But, as I have said, nothing.

I made spore syringes two ways: the first way was to boil the glass that I used to spray the water from the syringe into. The second way was your suggestion: to wipe it with alcohol. I did this all inside a glove box.

Most of my syringes I let hydrate for 24 hours, but the first one I did not. No difference.

Here are my working theories:

1.) Maybe there was still alcohol floating in the air inside the glove box that damaged the spores when I transferred them from the prints to the glass.

2.) Maybe the boiled tap water has chemicals in it which kill the spores when they are put into the syringe. (However, if that were true, shouldn't the water I used to mix the substrate also kill them when they are inoculated? Maybe not, because the jars are sterilized for an hour and a half, which may breakdown whatever chemical is preventing their growth. I don't now.)

3.) Maybe all of my prints were rendered nonviable somehow during the printing process. But if that were the case, not a single one should have produced growth, yet one did.

4.) God hates me.

The best I can come up with is to try making a liquid culture in a very unsanitary way, by simply scraping the spores directly off of a print into a jar of honey water. Maybe that will let me know if the issue has something to do with the syringes or not. The only other thing I can think of is to buy a bottle of distilled water, make syringes with that, and see if it makes any difference. I'll be experimenting with both.

Any help here would be greatly appreciated.
#53
PF - Tek / Re: Fruiting Chamber questions...
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#54
Cultivation / #100 TMC Oct 2013 Mushroom Jou...
Last post by fmrc - October 22, 2013, 04:28:55 PM
The #100 TMC Mushroom Journal for October 2013 is posted up for free read and inspection.  Go to www.mushroomsfmrc.com and click "TMC Journals" off of the Main Menu.  Here are brief contents.  Enjoy.  Highest Regards, Stephen L. Peele, Curator FMRC

     Pendulous lichens.............................................................01
     Psilocybin use and its history...............................................02
     Brewer's yeast, Saccharomyces cerevisiae the official microbe...02
     Irish potato crop................................................................01
     Treating frogs with a bacterium.............................................01
     Truffle's black magic flavor..................................................01
     Dr. Pollock's Death............................................................02
     Cooking With Mushrooms...................................................04
     How Mushroom Spores Are Spread About The World...............05
Antidote For Amanita muscaria and Muscarine.............................07
FREE Mushroom Spore Print Sample SO26...............................07
Mushroom Quiz....................................................................08
Color Photograph of Lactarius indigo............................................09
#55
Cultivation / Re: pinning on the bottom of t...
Last post by mofoiam - October 18, 2013, 09:26:11 PM
Curious about this myself. I had mine do the same thing last week, flipped half of them, they all grew, but none of them are pinning again.
#56
Cultivation / Re: fruiting help
Last post by mofoiam - October 18, 2013, 09:23:17 PM
Update?
#57
General topics / Re: detailed photo I.D. reques...
Last post by mofoiam - October 18, 2013, 09:16:25 PM
Holy crap, no. Maybe the bolete. Don't eat any gilled mushrooms you didn't buy at the site out from a trusted friend. On mycology what you have there are known as LBM's, little brown mushrooms. They are akin to the DYC's in botony, damn yellow compositaes. There are too many of each that look to much like each other to visually identify. I'd guess galerina but for real, no, don't eat those and wash your hands. The other one, thee polypore, the spongey looking one, is probably edible. There are rules for eating boletes, mostly don't eat orange or yellow ones out one with orange or yellow pores or ones that bruise blue (lol) but they are one of the safer families. Think porcini.
#58
Cultivation / peanut meal nutrient?
Last post by mofoiam - October 18, 2013, 08:50:42 PM
Sooo... I guess I didn't do enough research before I started and ordered from spore store. However, all my spores arrived in three days and, I'm ashamed, also the premade jars. Surprisingly, all twelve colonized eventually. B+ strain, but they used some sort of Peanut meal nutrient tek. I can't find any info on it. Before you go there, I have already gotten a very small first flush, the biggest was about four inches, but now I'm wondering if I need to proceed differently because of the peanut meal thing. Any ideas?
#59
Cultivation / Re: It's taking to long...
Last post by nuubshroom - October 09, 2013, 12:48:34 PM
I am pretty sure the substrate begun fruiting. Anyway, I put my stuff in the fruiting chamber and hopefully everything is good.
Regards
#60
Cultivation / It's taking to long...
Last post by nuubshroom - October 09, 2013, 05:55:00 AM
Hi guys!
This is what I've done

I put the inoculated substrate in a plastic box.
About 4-5cm of substrate.
Then I put some soil-vermiculite mix above it, 1-2cm.
Once this was done I wrapped the box in some plastic foil, took a needle and made 5 hole, just so that the mycelium could breath, and placed the box in a dark room.


Temp: ~24-26c
The humid is high because the plastic foil is very wet, can't almost not see the soil.

Now it's time for the mycelium to break through the soil. Here is a good picture of what it shall look like http://wiki.magiskamolekyler.org/images/thumb/7/7d/Casings.jpg/800px-Casings.jpg

Now, 5 days later, almost nothing has happened. 2-3 small small lumps have appeared.

Now to the question!
Because I made holes in the plastic foil, do you think the mycelium is fruiting? Because it's suppose to be like the picture above and once that is done, it's time for the fruiting. The mycelium that is visible, it's very white and good so no contaminations.

Sorry for bad English, it's not my first language.
Regards