Fungifun - Forum

Mushroom cultivation => Cultivation => Topic started by: enviromentaladditive on November 23, 2004, 05:10:12 PM

Title: sclerotia sterilization
Post by: enviromentaladditive on November 23, 2004, 05:10:12 PM
Is it possible to sterilize jars of rye seed in a pressure cooker without tyvec or polyfill lids, could I use a hole in the lid with foil wrapped over it?
Title: sclerotia sterilization
Post by: IndiaShroomer on November 23, 2004, 10:13:02 PM
Yes, you can sterilize that way. You only want to be careful that the foil doesnt get loose when the pressure builds up, if the steam gets into the jar, the moisture content will be too high.
Title: sclerotia sterilization
Post by: enviromentaladditive on December 01, 2004, 04:23:58 PM
QuoteYes, you can sterilize that way. You only want to be careful that the foil doesnt get loose when the pressure builds up, if the steam gets into the jar, the moisture content will be too high.
[snapback]499[/snapback]

Does the lid need to be shut tight, should the hole be punched in the jar before or after pressure sterilization?
Title: sclerotia sterilization
Post by: IndiaShroomer on December 02, 2004, 02:01:30 AM
Quote
QuoteYes, you can sterilize that way. You only want to be careful that the foil doesnt get loose when the pressure builds up, if the steam gets into the jar, the moisture content will be too high.
[snapback]499[/snapback]

Does the lid need to be shut tight, should the hole be punched in the jar before or after pressure sterilization?
[snapback]506[/snapback]

lid needs to be shut tight, punch holes before you sterilize. If you try this after sterilization, you may end up introducing contaminants in your otherwise sterile substrate.