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Messages - PCCP

#21
PF - Tek / Re: What's my next mycelium move?
May 12, 2011, 02:49:19 AM
Cheers BabyBlue,
You may not of read my first post. I basically cooked the spores by mistake for a couple of hours. I won't fall into that trap again. One learns from mistakes  :rolleyes:

I believe the spore syringe I had was very thin any way but still got 1 out of 6 to germinate. The full colonization was from 1 of 4 inoc sites so I think 36 days aint too bad. If all 4 inoc points were good then it could of been done in 9 days + germination time. I'll aim for 14 days next time round.

The LC tek was to safe guard propagation in case fruiting fails as I can't afford spores every failure.

My temp is now well in the comfort zone (26C tops) but is still essential as my night temp naturally drops to 8C (I live on a boat so insulation isn't house standard)

Thanks for your feed back.

:ph34r:
#22
PF - Tek / Re: What's my next mycelium move?
May 11, 2011, 02:16:27 AM
Day 47 since inoculation.

36 days of myc growth after 11 day germination.


I used my last 2cc of spores on 2X LC medium (malt dextrose 4% solution) 1cc each. Glass in bottom for agitation and each jar half full.

Injected 30cc malt dextrose 4% solution – extracted 5cc for a 3rd seperate LC from the only cake. It was left pretty wet and bruised.

I figured 3 LC's was a better risk than 1.

It was so dry it soaked the lot up. I really struggled getting any back out.
I wouldn't recomend this tek if your cake is really dry as I had to put more in than I wanted so it would give some up on return.

Everything returned to incubate for a few days, cake to recover etc. Agitating LC daily.

A stressful success so far.

:ph34r:





#23
PF - Tek / Re: What's my next mycelium move?
May 09, 2011, 03:01:54 AM
Day 45 since inoculation.

34 days of myc growth after 11 day germination.

Fully colonized the main body with v. very thick dense white, shrunk away from glass, only the bottom 5mm that's dry has failed. 25-27C

I'm stocked. Didn't think I would get this far. I've inverted the jar to help clear any CO2 while I prepare my LC jars over a couple of days.

I'm going to attempt an LC extraction with the idea it will help lost water back into the Jar also with what ever moisture is left behind.

I think after the hot start and delayed germination I may have some heat tolerant mycelium on my hands.

  :mellow:
#24
PF - Tek / Re: Over Heated Incubation??
April 27, 2011, 01:23:03 AM
Quote from: PCCP on April 13, 2011, 01:50:43 AM

2) Break of all uncolonized brf and try and grow a single shroom from there.


Cheers Vandal,
This option has always been in my armory, but as I wait and try to be patient to see what the best move is gonna be as the myc is still growing this could be the one

It's still growing so I'm still hanging, waiting and thinkin'  :ph34r:
#25
PF - Tek / Re: What's my next mycelium move?
April 24, 2011, 02:31:36 AM
Day 30 since inoculation.

19 days of myc growth after 11 day germination.

11.5cm size across, v. very thick dense white, shrunk away from glass, 25-27C

Didn't think I would get much more growth but I think its possible now. There are still moist areas to colonize but the dry parts are becoming loose and clearly visible mainly the 5mm base.

I'm going to attempt an LC extraction with the idea it will help lost water back into the Jar also with what ever moisture is left behind.
#26
PF - Tek / Re: Average time for colonization
April 20, 2011, 02:03:07 AM
Well done.
Now let it do it's thing. Check on its progress every 4 days or so. -_-

Keep an eye on those temps and when you do check in on those lovelies keep an eagle eye for contams  :wacko:. Anything not white is probably dodgy and at the upper end of the temp scale could easily run riot over the myc.

There's an argument for keeping temps down in the mid 70s where the contams don't thrive so much, as your myc develops and grows it will become safer as it moves toward 100% colonization. You can successfully keep temp low or it can be warmed up gradually until upper limit is reached at 50-75% colonization.

It may take a tad longer but will reduce contamination ratios a lot.

:ph34r:
#27
PF - Tek / Re: What's my next mycelium move?
April 19, 2011, 08:22:22 AM
It's day 25 since inoculation but germination didn't happen for 11 days.

What I have is 14 days of myc. growth. I think I can push it a little further but I need to add moisture soon or it may just eat the nutrients and leave nothing for any potential flush.

If I add water to it to help hydration I may also be introducing contams but it could be the thing that helps it to 100% colonization. I may also get an LC to continue propagation.

I need some experienced help on this.

???

Hanging in there... :ph34r:
#28
PF - Tek / Re: Average time for colonization
April 18, 2011, 05:06:32 AM
You should expect full colonization in about 14-21 days. You have a better idea once germination is reached.

I'd drop your temp too a little.

You're glass jar retain some heat and once the mycelium begins to grow it gives off heat too. This can be as much as 4 degrees.

So at 83f you might well be going up to 87f. 86f is optimal growth temp and growth slows considerably after that.



Clip it back a little 82f max if your confident, 80f if your experiencing fluctuation. 

:ph34r:
#29
PF - Tek / Re: What's my next mycelium move?
April 18, 2011, 04:51:46 AM
24 days since inoculation.

4 inoc points in each jar.
1 out of 24 ionc points germinated.
This 1 point in my only successful jar has grown from the previous golf ball to 8 cm. This is more than 33% of the Jar. Very thick white.
The bottom of the Jar seems too dry to colonize.

My plan is to inject this jar with 20cc of sterile water, shake vigorously, then withdraw 10cc or what I can to start a LC.

With this idea I hope to hydrate my jar and at the same time continue the propagation of my only myc.

Is this a good injectable amount?
Will withdrawing fluid back impede any further colonization?
Should I inject only and leave to colonize more?

Any thoughts?

:huh: :mellow:
#30
PF - Tek / Re: Just started
April 14, 2011, 05:40:47 AM
Keep your temps mid 70's until your sure you can moderate your're incubation temp well.

Good Luck  :ph34r:
#31
PF - Tek / Re: Unknown factor
April 14, 2011, 05:34:43 AM
Out of all the factors i believe its a thin spore solution.

On a similar thread my over heated spores i think were thin.

After 3 full weeks i my incubator I have 1 of 24 inoc sites germinated. I just can't believe all other 23 were killed in the heat but they could of been.

You could increase your spore shot per jar from 1cc to 2cc and reduce your jars from 8 to 4 to increase your chance of germination if its cause by lack of spores.

I'm going to make a separate liquid culture LC with my last 2cc of spores to have another go. This will avoid the brf until i know its growing.

:mellow:
#32
PF - Tek / Re: Over Heated Incubation??
April 13, 2011, 01:50:43 AM
So 2 more days and it will be 3 weeks since inoc.

I have 1 out of 24 inoc sites that germinated . Its produced think white myc that has shrunk away from the jar and spread the size of a golf ball.

The bottom of the jars has dried due to the initial over heating.

This myc is very precious as its the only bit I managed to develope and bring this far.

I seriously dont think the jar will achieve 100% colonization.

What can I do from hear? :

1) Make the myc into LC
2) Break of all uncolonized brf and try and grow a single shroom from there.
3) Add sterile water to the cake with a syringe

Please help, any suggestions  :huh: ^_^

-_-
#33
Cultivation / Re: Growing on Paper
April 07, 2011, 08:23:32 AM
Oyster will grow very well on toilet roll

check out the link http://www.ncbe.reading.ac.uk/ncbe/materials/MICROBIOLOGY/oyster.html  :ph34r:
#34
PF - Tek / Re: Over Heated Incubation??
April 07, 2011, 12:15:59 AM
As I said in my previous post I was beginning to question the quality of the syringe it self.

It came from a mycology and mushroom company here in the UK. Due to questionable legality of selling spores they ask not to be contacted with regards to growing.

This made me think in retrospect that they could easily supply a very weak syringe and not have any comeback.  :huh:

If I do need more spores I won't be returning for there services.  :angry:

It was a Colombian strain.
It looked clear with slight tone.
I created an air bubble and then shook before each inoculation.

If I can get some LC going from 1 of the 6 Jar then that would be great.

I may use the last of the 2cc of spores to try LC from that angle too.

Thanks for the feed back Vandal

:mellow:
#35
PF - Tek / Re: Over Heated Incubation??
April 05, 2011, 11:20:51 PM
After 11 days I have white in 1 of 24 inoc points YEEEES.

Come on you whites

:ph34r:
#36
PF - Tek / Re: Over Heated Incubation??
April 04, 2011, 10:21:02 AM
Still no good stuff, but I got no bad stuff either.

I'm beginning to question the spores themselves.

I might test them on a single Liquid Culture Jar

:mellow:
#37
PF - Tek / Re: Over Heated Incubation??
March 31, 2011, 02:34:18 AM
Cheers Vandal,

I don't see white yet but I ain't giving up. :mellow:
#38
PF - Tek / Darkness
March 29, 2011, 06:59:53 AM
Should I keep my inoculated jars in complete darkness until 100% colonized or just normal day/ night cycle?

I look forward to anyone replying. I need some input.

Thanks
#39
General topics / Re: Hot Spore Solution
March 29, 2011, 05:13:28 AM
I've found out Cubensis mycelium growth slow considerably after 86f and reaches thermal death @ 104f. Is this also true for ungerminated spore solution?

Any help out there would be great for this first timer. -_-
#40
PF - Tek / Re: Over Heated Incubation??
March 29, 2011, 01:18:43 AM
No signs of white yet.

I'm using a timer with my incubator. Can't afford the thermostat just yet.

I've manage to stabilize my temp in the 22-26C range, difficult at night as my ambient temp plummets, but I think I can refine the temp band a little more with a few more tweaks.

I've been reading it's possible to salvage a less than fully colonized cake by cutting off uncolonized substrate, then crumble 'n' case.

Is this worth thinking about on my first grow since I only got 2cc of spores left. I wanna spend next months extra cash on a reptile hydro-thermometer, not more spores.

Any input is cool on how to manage my fuck up. :rolleyes:

Cheers Everyone :ph34r: