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Mushroom cultivation => Cultivation => Topic started by: jimboyia on March 29, 2012, 01:28:58 AM

Title: anti-biotics in agar culture?
Post by: jimboyia on March 29, 2012, 01:28:58 AM
Howdy,

got 3 different strains of spore prints (Golden Teacher, Mazatapec & Panaeolus) from Sporeworks. Problem is they were "inspected" by customs before being released to me. My concern is that these will be quite heavily contaminated spores now.

I can go off the PF tek & instead take option of plating out spores on Petri dishes and try get a reasonably pure culture from that but was wondering if I can introduce an antibiotic to take care of any nasty bacterial contamination. 

any ideas? Comments? Advice?

ta
Jim
Title: Re: anti-biotics in agar culture?
Post by: Vandal_xx on March 29, 2012, 04:52:37 AM
I would just use em and hope for the best. If there are contams then you are screwed either way. Give em a shot. Who kows.
Title: Re: anti-biotics in agar culture?
Post by: pincho on April 10, 2012, 11:11:25 PM
I am strongly against anti-biotics in the agar culture.
Title: Re: anti-biotics in agar culture?
Post by: jimboyia on April 14, 2012, 06:03:28 AM
OK, but why?