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Topics - spp.

#1
PF - Tek / A major f**up?
April 10, 2010, 11:31:18 AM

So there, today I was inoculating some PF jars when the syringe needle detached. I immediately wiped it with H202, sticked it back and continued inoculating tha jars, but now I'm worried if it wasn't enough. Basically I was working in a glowbox chiefly sprayed with peroxide, but hey shit happened and I'm afraid all my jars are doomed now. Has anyone had such an experience?
#2
Hello everyone. At the moment there are 3 jars inoculated 17 days ago with Transkei strain, kept at fluctuating 29-30.5 Celsius. The colonization started from bottoms of the jars and was slowly reaching the tops of them. Now they seem to be fully colonized except some upper spaces.  2 days ago I inverted the jars because there was too much water heavily condensing on the bottoms of the jars (this is my first time doing the PF tek and of course everything is a little bit messed up). This appears to have aided the issue with uncolonized upper areas a bit, but it's still going very slow. What could be the reason for the jars not wanting to colonize the upper parts of BRF/verm mix and how could I help it? Thanks in advance.