Fungifun - Forum

Mushroom cultivation => Cultivation => Topic started by: turbolacis on February 08, 2007, 03:29:57 AM

Title: Spore print!
Post by: turbolacis on February 08, 2007, 03:29:57 AM
Hi!

I am new to growing mushies, but very enthusiastic in growing my own! English is not my native language so excuse me for my mistakes! I love this forum and it's community.
I have searched a lot, but haven't found instructions or any topics regarding how to inoculate jars with spore prints! Could you post a little instruction how to do it, or a link to instruction. With wich strain you would suggest me to start? Thanks a lot.
Title: Re: Spore print!
Post by: anno on February 08, 2007, 05:09:34 AM
If you want to inoculate directly with a sporeprint, you would have to scrape spores directly on the sterilized substrate. This would preclude you from using a protecting vermiculite layer, and thus preclude from using the pf tek.

What you could use is http://www.fungifun.org/English/Grain-For-Simple-Minds but the contamination risk is quite much higher than using a syringe to inoculate pf jars.
Title: Re: Spore print!
Post by: pianoman on May 21, 2009, 12:53:06 PM
Hey dude in a glove box scrape print into distilled water and draw up into steril syringe if not mistaken
Title: Re: Spore print!
Post by: MT on May 21, 2009, 02:02:16 PM
Hey dude, no one has posted on this thread for over two years (except for you ), and now me.
Title: Re: Spore print!
Post by: psilocybin warrior on May 21, 2009, 06:15:56 PM
HA-HAW!

PW
Title: Re: Spore print!
Post by: veda_sticks on May 23, 2009, 09:55:33 PM
Quote from: anno on February 08, 2007, 05:09:34 AM
If you want to inoculate directly with a sporeprint, you would have to scrape spores directly on the sterilized substrate. This would preclude you from using a protecting vermiculite layer, and thus preclude from using the pf tek.

What you could use is http://www.fungifun.org/English/Grain-For-Simple-Minds but the contamination risk is quite much higher than using a syringe to inoculate pf jars.

Grains are no more contaminant prone than pf tek jars. Using that tek however could lead to higher contamination rates.

Grains should be pressure cooked for 90 minutes at 15 psi, not 60. Also, that method of preparing grains is old.

http://www.mushroomvideos.com/Grain-Preparation

Best rye grain tek IMO, prefect moisture content everytime.

The pf tek does not need to have the dry verm layer. You can leave out the dry verm and instead use a filter just as you would a grain jar, using tyvek for instance.

The disadvantage of using spores from a spore print, is it may take longer for your spores to germinate as they will need ot hydrate sitting ontop of your substrate.