Fungifun - Forum

Mushroom cultivation => Cultivation => Topic started by: spaceboi on February 08, 2010, 02:42:56 PM

Title: what is going wrong
Post by: spaceboi on February 08, 2010, 02:42:56 PM
I am confused. My first batch of jars i innoculated are going great. They have a half inch of perlite on top accidently instead of vermiculite. They were even sterelized in a crappy pressure cooker without the rubber seal in the lid. i ordered the spore syringe from the hawks eye.
Now the problem. Been using a spore print and making own syringe. Even bought a new pressure cooker. But all have been molding since. Our incubating container is a fish heater in four inches of water with another plastic containter to hold the jars. there are little droplets of water inside of the jars. Constantly 85 degrees F and 32% humidity.
for steralizing the syring i boiled water for ten minutes sucked it up in the syring 4 or 5 times. waited 2 hours for it to cool off then i went in to a stireal room under the stiairs i cleaned entirley with clorox and then spraid the room with lysol. if i dont wait long enought for the lysol to settle can it do anything to the prints?

another thing i noticed was when i made up my srying and let it set for 18 hours they didint collect  together like the one i had goten already made up.
Title: Re: what is going wrong
Post by: dub504 on February 08, 2010, 03:21:20 PM
You should probably come down a few degrees, right around 80. You're incubating your syringes?
Title: Re: what is going wrong
Post by: spaceboi on February 08, 2010, 03:23:17 PM
there B+ thought it was suppsed to be at 85?
Title: Re: what is going wrong
Post by: dub504 on February 08, 2010, 03:26:02 PM
The mycelium will create heat when colonizing.
Title: Re: what is going wrong
Post by: spaceboi on February 09, 2010, 12:09:54 PM
no iv been just leaving them in my room in a box should i be? and how can i collect the live mycelium from the jars so i can inoculate new jars?
Title: Re: what is going wrong
Post by: moorhS on February 09, 2010, 12:57:35 PM
i'm not sure about doing a g2g transfer with brf don't think you can  :huh:
Title: Re: what is going wrong
Post by: dub504 on February 09, 2010, 02:20:32 PM
http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek (http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek)
Title: Re: what is going wrong
Post by: moorhS on February 09, 2010, 05:13:06 PM
huh nice dub never seen that
Title: Re: what is going wrong
Post by: dub504 on February 09, 2010, 07:07:25 PM
I tried it a while back, and it does work.