Menu

Show posts

This section allows you to view all posts made by this member. Note that you can only see posts made in areas you currently have access to.

Show posts Menu

Messages - timewave

#1
PF - Tek / Tried twice and wasted 2 syringes
February 13, 2005, 05:28:10 AM
I have to say the syringes were just pretty weak.  I took the second syringe i received and made some liquid mycelium and innoculated 12 more jars none of the jars germinated but the liquid mycelium did.  I then took the liquid and innoculated 4 more jars of liquid then those same 12 jars and everything is now running perfect :D  My first caseing
#2
PF - Tek / Tried twice and wasted 2 syringes
December 17, 2004, 09:08:50 AM
QuoteThe only way the PC could mess things up waould be if it didn't sterilise the substrate (obviously not the case due to no contam), or if the process dried the jars out - though this also seems unlikely.

Seems very odd that this happened to two syringes. Could yo see evidence of spores in the syringes?


When you say the jars didn't smell, have you actually opened one up fully to investigate yet?
[snapback]541[/snapback]

I didn't look that cloesly but I will this time, 3 days ago I ordered a syringe and waiting for it to show up I figure this would be my last attempt and ill work at growing other stuff.

Now if I created 10 jars brf pc'd them and they have been in a box for 3 days tomorrow the syringe should be here can i still use the jars or should i toss them and make new ones?
#3
PF - Tek / Tried twice and wasted 2 syringes
December 16, 2004, 06:05:55 PM
QuoteSounds like the syringes are not viable. Are they old?
[snapback]538[/snapback]

No I just purchased them and used them the next day.


I figured it was the pressure cooker nukeing  them since I figured the spores should be viable.  I just need one darn jar to colonize!

#4
PF - Tek / Tried twice and wasted 2 syringes
December 15, 2004, 08:09:55 PM
Ok, pretty much I can't get PF jars to colonize.. I'm starting to think my pressure cooker is killing the jars.  I clean everything and use 1/2 cup verm 1/4 cup brf 60 cc's of water... Mixed up and made 12 jars.. Covered the 4 holes with electrical tape and the middle hole filled with polyfill.  I then pressure cooked them for 40 minutes at 15 PSI.  Now my pressure cooker has a weight on it that doesn't move.. I brought the heat up until steam came up then put the weight on brought the heat to a little under medium and waited.  The water inside the pressure cooker was boiling I could here it splashing around.  Is that right?

Anyways After the jars cooled over night I inoculated the jars 1 cc each.

I used the rubbermaid tub with a 50 watt thermo in it.. checked the jars 40% humidity and 83 degrees.   Waited two weeks nothing!

I did this exact thing twice the jars don't smell so it doesn't seem like contaiminated.  I just don't see anything growing.

This is very discouraging I should of odered more syringes ahead of time :(..  Any advice is appreciated.. Thanks