Fungifun - Forum

Mushroom cultivation => Cultivation => Topic started by: tenaglia on April 04, 2009, 02:57:51 AM

Title: Agar cleaning process
Post by: tenaglia on April 04, 2009, 02:57:51 AM
How do you obtain a clean culture? I tried to select apparently clean mycelium to inoculate a new petri dish, but I always carry some pennicillium or something like that; should I bath the wedge in a peroxide solution? I thought It shouldn't be neccessary   :huh:
Title: Re: Agar cleaning process
Post by: psilocybin warrior on April 04, 2009, 08:21:57 AM
Did you boil your potatos first? if not, boil them instead of the peroxide dip.


PW
Title: Re: Agar cleaning process
Post by: tenaglia on April 04, 2009, 08:26:09 AM
I use ready made potato dextrose agar, I sterilize it for 20 minutes then I leave the fresh dishes in the incubator for 4-5 days to see if they are poured clean.
Title: Re: Agar cleaning process
Post by: psilocybin warrior on April 04, 2009, 08:33:36 AM
Wait, I dont think I understand.... your not innocing them right after sterilization but waiting 4-5 days?
Title: Re: Agar cleaning process
Post by: tenaglia on April 05, 2009, 04:58:47 AM
Right, I dissolve 39g potato-dextrose with 5g agar in one liter water, I adjust the ph, I sterilize it for 20 min, I pour it in 20 dishes, I wait a couple of days to see if I worked sterile enough, then I innoculate them :ph34r: If you do not wait, you have chance to innoculate a petri which isn't sterile anymore...

The biologist I work with told me to do so, he works with tissue-culture (orchids, meat-eaters, aquarium plants and much more)
Title: Re: Agar cleaning process
Post by: psilocybin warrior on April 05, 2009, 01:28:45 PM
I've never bothered waiting whats steril is steril, maybe the waiting a few day's is allowing it to produce another kind of bacteria, its cool ur working with a biologist, however this is mycology not biology.

Pw