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Topics - PCCP

Pages: [1]
1
PF - Tek / Early BRF cakes. What should I do?
« on: May 24, 2011, 02:55:55 AM »
I've made a new batch of BRF cakes but realised I won't be ready to inoc for another 6-9 days.

Do I refrigerate now then sterilise?

or

Sterilise now then refrigerate and bring to room temp before inoc?

Thanks

 :huh:

2
Cultivation / Liquid Culture growth
« on: May 12, 2011, 07:57:09 AM »
I'm growing 3 liquid cultures.

Extra Light Malt and Dextros
6 tsp sugars in 750ml solution (4% = 30g = 720ml)
2g sugar per 1 point mark on brewing hydrometer in a 750ml bottle
4% solution = 15 points SG:1.015

3 Jars half full of medium + glass shards.
Steam boiled for 30 mins
2 jars inoculated with 1cc of spores each
1 jar inoculated with fluid injected then withdrawn from full colonized PF cake

2 small piece of broken glass were added to the jar before sterilization. Agitating daily to cut up the mycellium which can form into an unsuckable clump in the jar

These are my questions:

The medium is a light brown. Will the growth still be white?
As the myc grows does it naturally fall to the bottom of the jar in between agitation or should it be more floaty?

Thanks

 :mellow:



3
PF - Tek / Darkness
« on: March 29, 2011, 07:59:53 AM »
Should I keep my inoculated jars in complete darkness until 100% colonized or just normal day/ night cycle?

I look forward to anyone replying. I need some input.

Thanks

4
General topics / Hot Spore Solution
« on: March 27, 2011, 02:50:59 AM »
What temperature can P.Cubensis spore solution tolerate before its doomed? :huh:

5
PF - Tek / What's my next mycelium move? Get Out of Jail Tek
« on: March 26, 2011, 01:37:28 AM »
Hi everyone, complete noob here.

Have read and re read the PF-Teks and understand fully what I should of done but I had a major error in judgement.

I've inoculated 6 brf jars with 1.3cc of spores each and put them in my incubator that normaly gets to 28C max during the night, unheated through the day. My place is average 15C so extra heat is needed

I put them to bed and thought I would add a layer of tin foil to keep in complete darkness. I then had to leave.

When I got back 5 hours later the air temp was nightmare 35C. I didnt realise the foil would retain so much extra, as 7C deg. I opened and cooled of straight away and only closed for the night when it had dropped to 25C. This morning was down to 18C

I'm not sure how long the heat was at 35C testing the heater I think it was about 2 hours.

Is 2 hours at 35C too much for freshly inoculated jars?

Will they germinate?

If they germinate are they doomed for less than full colonization due to moisture loss in the early over heated hours?

I should wait to see at least  germination and keep the temp low say 20-22C and humid to prevent any more moisture loss?

Sorry for the long post, just wanted to give more experienced people all the facts.

 Peace :huh:


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