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Topics - g fung

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Cultivation / making injection port
« on: November 08, 2009, 06:44:18 AM »
anyone got any advice on how to make rtv silicone stick to spawn bags without pealing off?

Cultivation / Problems with bags
« on: August 13, 2009, 09:04:27 AM »
I have never had a problem like this and I'm very frustrated.

This is my usual setup: I put about 6 quarts of soaked and drained grain in a spawn bag. I impulse seal the bag(s).  I then pressure cook it from 1 to 1.5 hours (depending on whether I have 1, 2 or 3 bags in the cooker). I inoculate through an injection port. I Then put the inoculated bags into my incubator setup which is a full size hotel steam pan that floats in another steam pan with a fish tank heater set at 86.

I have just lost 3 bags. This really irritates me because while I have had a bag here or there go bad, I have never had something like this go wrong. The bags started strong as usual but have now been tainted by green mold after 25% colonization.

I typically use bags from myco-supply but this time I was forced to get them from sporeworks. The bags seem to have a thinner filter patch. I was wondering if the condensation on the bags hit the filter patch and dripped down to introduce the contam. 

What is the prevailing opinion on shaking bags?  And for those who approve what is your procedure? I am a fan of the shake and would like to know your two cents. This is my biggest suspicion. The spawn that may have come into contact with the filter patch, during the breaking up and mixing of the spawn, was compromised and thus compromised the bag. Another thing that I considered is that the handling of the bags during the shake punctured the now pressure cooked and brittle bags.

Cultivation / Lost recipe
« on: July 21, 2009, 10:04:48 AM »
I once ran across a cool recipe for in vitro spawn bags. The recipe was demonstrated with p.e. It had all kinds of good stuff in it oils, oyster, grain, poo, etc. Does anyone out there know its location?

Cultivation / Smell of Wrong?
« on: February 01, 2009, 05:14:22 PM »
I spawned some fully colonized popcorn to a 80:20 dehydrated poo and coir (and re hydrated and pasteurized). The spawned mix is in full size stainless hotel steam pans. The pans are in garbage bags. It has been a week and there is a strange smell almost like ammonia and the bubbles from a carbonated drink. This is the first time I have used these pans but they are sturdy and relatively cheap on the interwebs. What do you guys think? Has something gone wrong or is this new container just exposing me to a smell that the containers I have used in the past did not boast?

Cultivation / Strange arrival of knots
« on: October 29, 2008, 08:12:02 PM »
I have a penis envy grow going. It is cased with vermiculite, the bulk substrate is cow poo, the spawn was wild bird seed inoculated with a multispore syringe.

The "problem" is that the multitude of knots did not arrive until the second flushes beginning?
The first flush looked slim when I saw just a few pins and no more after that. As expected the crop was horrible. I blamed it on being a multispore and not an isolate, as I have heard that it is especially well worth the effort and possibly necessary with this particular strain (P.E.). Now that the trays are due to flush again I am seeing a tray full of knots.

Any thoughts as to why?
...At least any thoughts other than fungus just does what it wants to sometimes.

Cultivation / Mycological Peptone and Antibiotics
« on: October 15, 2008, 04:37:34 PM »
What exactly are the practical uses of mycological peptone and antibiotics? I have seen the antibiotics somewhere but I can't seem to find them again. Any thoughts?

Cultivation / Multi Spore Liquid Culture With Pan Cyan
« on: October 10, 2008, 04:55:18 PM »
I have read that the pan cyan mycelium is not as robust as cube mycelium. Now I am wondering what I am to expect with my multispore liquid culture (4% honey 96% water). In other words, how hard is it to tell when you are good to go with the formentioned parameters?
 Thank you.

PF - Tek / How important is vermiculite under my circumstances
« on: September 21, 2008, 07:05:48 AM »
So I am typically all about skipping any step that I can get away with. My method is to start with birdseed jars and then case in composted poo and verm. If this is my method do I really need vermiculite mixed into my cake? My thinking is that it is wasting space that could be used for nutrients and for more mycelium which would then translate into quicker stronger colonization of the casing. Am I right?

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