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General topics / refrigerated grain spawn
« on: January 30, 2009, 06:39:59 AM »

How long can colonized rye grain spawn last in the fridge before it is no longer viable for 1. g to g transfers 2.Spawn to bulk 3. case and fruit the grain.  Is there any one of the three where it can last longer?

Thanks

2
General topics / substrait preservation
« on: January 28, 2009, 09:11:21 PM »

Can pre pasteurized bulk sub's be frozen for semi-long term storage? (1-2months) Once thawed wouldn't it be relatively sterile w/ all beneficial microbes being killed?  Could one PC the sub after it was thawed or would the quality degradation be too significant?  My supplier sells only 10 lb. increments leaving me a lot of waste.   

Thanks you guys are a big help!

3
Cultivation / clone
« on: January 18, 2009, 10:55:38 AM »

How many times can a sub-strain be cloned before problems occur?
I have been using the same culture for almost a year being rather careless about keeping a master Jar and too lazy to make a slant.  A jar from a jar to a jar to jars to a jar kind of a deal, and now I am starting to see deformed shrooms.  The strain still performes well however. Is it too late to make a slant?
Thanks
 

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