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anyone got any advice on how to make rtv silicone stick to spawn bags without pealing off?

I have never had a problem like this and I'm very frustrated.

This is my usual setup: I put about 6 quarts of soaked and drained grain in a spawn bag. I impulse seal the bag(s).  I then pressure cook it from 1 to 1.5 hours (depending on whether I have 1, 2 or 3 bags in the cooker). I inoculate through an injection port. I Then put the inoculated bags into my incubator setup which is a full size hotel steam pan that floats in another steam pan with a fish tank heater set at 86.

I have just lost 3 bags. This really irritates me because while I have had a bag here or there go bad, I have never had something like this go wrong. The bags started strong as usual but have now been tainted by green mold after 25% colonization.

I typically use bags from myco-supply but this time I was forced to get them from sporeworks. The bags seem to have a thinner filter patch. I was wondering if the condensation on the bags hit the filter patch and dripped down to introduce the contam. 

What is the prevailing opinion on shaking bags?  And for those who approve what is your procedure? I am a fan of the shake and would like to know your two cents. This is my biggest suspicion. The spawn that may have come into contact with the filter patch, during the breaking up and mixing of the spawn, was compromised and thus compromised the bag. Another thing that I considered is that the handling of the bags during the shake punctured the now pressure cooked and brittle bags.
 

I love popcorn because of its all around ease of use. Its cheap and easily procured without too much suspicion from the local garden store that already has me marked down as a sketch-ball. Its relatively clean to begin with. It doesn't make a mess by spreading everywhere and sticking to everything like millet. Then when it comes time to mix with the bulk substrate it breaks up very well into single kernels.
Im not sure that any claims that the corn is quick are correct though.

yeah, dub got it right. that was the misunderstanding.

I just sterilized two big bags of rye grass seed (not berries) to see what happens with cubes and cyans

I see some nice healthy myc growing. so far so good. why exactly is corn not supposed to work?

I once ran across a cool recipe for in vitro spawn bags. The recipe was demonstrated with p.e. It had all kinds of good stuff in it oils, oyster, grain, poo, etc. Does anyone out there know its location?

I just did. Didn't think to check. Ill let you know how I make out.

I think that the sub was too wet. I chucked it just to be safe.

I spawned some fully colonized popcorn to a 80:20 dehydrated poo and coir (and re hydrated and pasteurized). The spawned mix is in full size stainless hotel steam pans. The pans are in garbage bags. It has been a week and there is a strange smell almost like ammonia and the bubbles from a carbonated drink. This is the first time I have used these pans but they are sturdy and relatively cheap on the interwebs. What do you guys think? Has something gone wrong or is this new container just exposing me to a smell that the containers I have used in the past did not boast?

dude, you couldn't wait?

It def was the second pic. Most the pins fruited. And now a good portion, 25%, look as though they are about to abort There is no trich. I just can't get my head around the second flush being this much more vigorous and plentiful (which is the question afterall)   Could it be that I blew it on the PH?

Its not trich. Its just a sea of second flush pins after a really crappy first flush.


?

I have a penis envy grow going. It is cased with vermiculite, the bulk substrate is cow poo, the spawn was wild bird seed inoculated with a multispore syringe.

The "problem" is that the multitude of knots did not arrive until the second flushes beginning?
The first flush looked slim when I saw just a few pins and no more after that. As expected the crop was horrible. I blamed it on being a multispore and not an isolate, as I have heard that it is especially well worth the effort and possibly necessary with this particular strain (P.E.). Now that the trays are due to flush again I am seeing a tray full of knots.

Any thoughts as to why?
...At least any thoughts other than fungus just does what it wants to sometimes.
 

If you have grown with casings before or you are just up for a bit more of a challenge you could give the pan cyans a twirl. I just started playing with this species and so far so good. The mycellium rips through substrate and the growth parameters are the same as cubes.

Impatient as usual! I got wisps! Looking forward to baby jellyfish.

Wow, that is MEAN looking!
Aspergillus Niger is less likely to cause human disease than some other Aspergillus species, but if large amounts of spores are inhaled, a serious lung disease, aspergillosis can occur. Aspergillosis is particularly frequent among horticultural workers who inhale peat dust, which can be rich in Aspergillus spores. Less commonly, it has been found on the walls of ancient Egyptian tombs and can be inhaled when the area is disturbed.[citation needed] A. niger is one of the most common causes of otomycosis (fungal ear infections), which can cause pain, temporary hearing loss and, in severe cases, damage to the ear canal and tympanic membrane.

I worry about any heat at all, as I worry about the heat speeding the oxidization process and reducing potency. If your climate is really dry, a loose stack or pile of shrooms with a big fan on it works provided that you remember to rotate and fluff the shrooms. Thats only if your really dry mind you. My climate is usually about 70%rh to 80%rh so this method is out for me but it has worked for me when I have lived in other regions.

I prefer desiccant in a container with a fan in it. Cheap, easy and ultra effective. Another side bennifit to this is that while you are purchasing desiccant you can also purchase O2 absorbing packets and use the two together to preserve the fungus and its miraculous chemistry. Desiccant can be easily found on the web in bulk (loose beads in cans or big jars and not in the little white packets), for cheap (with shipping you can probably get it for about $35).  I would order some desiccant today, return your dehydrator tomorrow, keep a fan on em in the meantime.

Silica gel and some other varieties can also be renewed to their full potential by cooking it in the oven, but check the manufacture's specs and instructions before doing so. Indicator desiccant is SWEET!

8 days and I noticed some tiny tiny floaters. It is hard to determine whether or not it is mycellium, spores or just debris suspended in the solution. Keep in mind that the bird seed that I inoculated 9 days ago with the same syringe are 75% colonized. Next time I will try several nutrient sources (in separate containers with each formula duplicated) and peptone in the duplicates of the formulas. Peptone is after all only $20.

I have my doubts on the peptone though. If the spores don't sprout then the peptone can't do anything other than stimulate an unwanted secondary organism.

Also, I will try to clone to a reliable LC to eliminate the any variables associated with innoculateing with a multispore suspension.

Any thoughts or suggestions are welcome and appreciated.

That site is fantastic!

Thank You!